Vigilância molecular como ferramenta para detectar Brucella spp., em leite e queijos artesanais no Rio Grande do Norte, Brasil.
Vigilância molecular como ferramenta para detectar Brucella spp., em leite e queijos artesanais no Rio Grande do Norte, Brasil.
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DOI: https://doi.org/10.22533/at.ed.9252404069
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Palavras-chave: queijo de coalho; leite in natura; Brucella spp; alimento seguro; saúde única.
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Keywords: Coalho cheese; raw milk; Brucella spp; safe food; One health.
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Abstract: Introduction: A study was carried out to characterize the epidemiological situation of the pathogenic microorganism Brucella spp. in raw milk and artisanal Coalho cheese from family cheese-producing agro-industries in Northeast Brazil. Animal products, such as dairy products and their derivatives, can contribute to the development of diseases in humans; especially when obtained from infected animals and that were not thermally treated. Bacteria of the genus Brucella spp. represent a biological hazard and with great importance for One health. Methodology: 18 samples of raw milk and 18 samples of coalho cheese were sampled, observed by polymerase chain reaction (PCR) with a generic primer for Brucella spp, forward 5'-TCAGGCGCTTATAACCGAAG-3' and reverse 5'- ATCTGCGCATAGGTCTGCTT-3'. Results: DNA from bacteria of the genus Brucella spp. was not found in these 18 samples for raw milk and 18 samples of Coalho cheese. Discussion: The non-detection of DNA in the samples surveyed is attributed to suggestive factors, such as: most of the animals already have been tested for brucellosis (Rose Bengal screening test) with non-reactive results; and, also, due to the fact that the state of Rio Grande do Norte registers very low anti-brucella immunization (≤ 5%) until 2018, generation in which the current lactating cows are found. Conclusions: The negative result of the tests does not reflect the real epidemiological situation of bovine brucellosis in Rio Grande do Norte; however, assure the quality of the milk and coalho cheeses tested, which can be considered safe in relation to the researched bacteria.
- Clebert
- Giovanni Brito Medeiros
- Pirajá Saraiva Bezerra Neto
- Denize Monteiro dos Anjos
- Maria Luana Cristiny Rodrigues Silva;
- Sérgio Santos de Azevedo,