Resultados de la evaluación in vitro de la actividad antibacteriana de los extractos acuosos de las semillas de Coffea arabica L. y Persea americana Mill, sobre Salmonella typhi aislada de los manipuladores de alimentos en la Ciudad Universitaria de Perayoc - UNSAAC. 2017

• DOI: https://doi.org/10.22533/at.ed.5342406098

• Palavras-chave: Actividad – antibacteriana, extractos - semillas - Coffea arabica L. - Persea americana Mill, Salmonella typhi, manipuladores – alimentos

• Keywords: Activity - antibacterial, extracts - seeds - Coffea arabica L. - Persea americana Mill, Salmonella typhi, handlers - food

• Abstract: The seeds of Coffea arabica L. and Persea americana Mill are used in traditional medicine for diarrheal processes, but they needed to be investigated, so their antibiotic action against Salmonella typhi was quantified in vitro, and it was considered to isolate said microorganisms from handlers of food who worked in the kiosks of the university city of Perayoc, because many of the diners reported having diarrheal problems. The research problem was: What is the antisalmonella typhi effect of the aqueous extracts of the seeds of Coffea arabica L. and Persea americana Mill? The objective was to evaluate in vitro the antisalmonella typhi activity of the aqueous extracts of the seeds of Coffea arabica L. and Persea americana Mill. The type of design is quasi- experimental, prospective and quantitative. Scope of study: All the restaurants and kiosks of the University City of Perayoc that wanted to participate freely were worked on. Sample: for stool cultures, 32 samples were used. The aqueous extracts were prepared at different concentrations. The latest in vitro microbiological techniques according to NCCLS (20) will be used, both for stool cultures and for antibiograms. It is concluded that: the prevalence of Salmonella typhi was 60.42%. In the ethanolic extract of 70% of coffee and avocado seeds, the presence of steroids and triterpenes, phenolic compounds and alkaloids (+++) and the absence of saponins, flavonoids and tannins were determined. The working hypothesis is confirmed which is that thee aqueous and ethanolic extracts of 70% of the seeds of Coffea arabica L. and Persea americana Mill, show intermediate in vitro activity on Salmonella typhi strains isolated from food handlers at the University City of Perayoc - UNSAAC. Due to the diameters of the inhibition halos of the aqueous and ethanolic extracts of 70%, these extracts are considered antibacterial agents.