UM NOVO PROTOCOLO NA ETAPA DE DESIDRATAÇÃO E HIDRATAÇÃO NA ROTINA HISTOLÓGICA, UTILIZANDO ÁLCOOL COMBUSTÍVEL

• DOI: 10.22533/at.ed.4471925098

• Palavras-chave: Histologia, etanol, intestino delgado, hematoxilina, eosina.

• Keywords: Histology, ethanol, small intestine, hematoxylin, eosin.

• Abstract:

  The objective of this study was to analyze duodenum sections of Felis

  catus domesticus L. by using fuel alcohol in the histological routine. The material

  was processed in the Laboratory of Histological and Embryological Techniques of the

  Institute of Biological Sciences (ICB / UPE), with the approval of the Committee for

  Ethics in Animal Use (CEUA / UPE: 002/2017). The collected tissues were fixed in 10%

  buffered formalin for 24h. After fixation, tissues from the treated group were dehydrated

  with Petrobras BR ® alcohol in a series of increasing concentrations between 70%

  - 100%, clarified in xylol and embedded in paraffin. The Leica® RM2165 microtome

  was used to make 5 μm thick slices. The slides were stained with Hematoxylin / Eosin

  and the alcohol used in the staining and assembly was the BR® alcohol. The protocol

  of the control group was based on the routine histological technique with alcohol P.A.

  The slides obtained were photographed using Olympus SC30 camera coupled to an

  Olympus CX31 trinocular optical microscope. The results show the small intestine of

  Felis catus domesticus where it is possible to identify all the constituents of the mucosa,

  submucosa, muscular and serous layers. It can be concluded that alcohol BR® did not

  cause alterations that compromised the histological quality of the analyzed tissues.

  It can be used in all stages related to the preparation of histological preparations,

  especially when the stage requires concentration below 95oGL.

• Número de páginas: 15