THE SYSTEMATIC INVESTIGATION OF L-ASPARAGINASE PRODUCED BY FILAMENTOUS FUNGI

• Palavras-chave: Atena Editora

• Keywords: Asparaginase. Anti-neoplastic. Enzyme. Lymphoblastic leucemia.

• Abstract:

  L-Asparaginase is an antineoplastic enzyme agent, used in the treatment of lymphoblastic leukemia. Various microorganisms such as bacteria, fungi, plants, and yeasts are able to produce L-asparaginase. Current, the most commonly used L-asparaginase against leukemia comes from bacteria. However, L-asparaginase from bacteria can cause hypersensitivity in the long-term use, leading to allergic reactions and anaphylaxis.  For this reason, in the last five years our group has been engaged in discovering L-asparaginase producing eukaryotic microorganisms, in order to obtain an enzyme that possibly causes less adverse effects in humans. To this end it has been used a simple and rapid assay method for the detection of L-asparaginase production from different filamentous fungi using phenol red as a detector of the activity for this enzyme by the formation of a pink zone in solid medium, at different pH and temperature conditions. In the present study, 10 fungi strains were analyzed, and 5 were selected and identified as L-asparaginase producers. The best producer strains were Peacilomyces variotii, showing activity reached 17.8 U/mL after 48h at 33 °C under experimental conditions.

• Número de páginas: 15