THE IMPORTANCE OF IN VITRO TESTS FOR BIOMATERIALS AND DRUGS APPLIED IN THE MEDICAL AREA

• DOI: 10.22533/at.ed.61520240114

• Palavras-chave: Atena

• Keywords: cell culture, in vitro techniques, biomaterials, cytotoxicity, genotoxic

• Abstract:

  Introduction: New biomaterials and molecules have been widely studied to improve the quality of people's life. However, they should have characteristics that ensure their biocompatibility. Therefore, in vitro assays are performed to prove the absence of cytotoxicity and genotoxicity. Objective: To review the literature in order to present the most used in vitro cytotoxicity and genotoxicity tests, their applications and specificity, as well as the cell cultures involved in these methods. Methods: A review of the literature with articles, regulations, dissertations and theses was carried out to describe the most used in vitro biocompatibility (genotoxic and cytotoxicity) tests in research with new molecules and biomaterials. Results: The most used cytotoxicity tests are MTT, MTS, XTT, nitric oxide, Alamar blue and neutral red. The most commonly used genotoxic tests are comet and micronucleus assays. Conclusion: Different biocompatibility tests can be used with different types of cell cultures, since they demonstrate that the cells are viable, either by cell proliferation or the production of some specific metabolite.

• Número de páginas: 13