TAXA DE GESTAÇÃO DE HEMI-EMBRIÕES CAPRINOS TRANSFERIDOS

• DOI: 10.22533/at.ed.26319120910

• Palavras-chave: bissecção, transferência de embrião, caprino

• Keywords: bisection, embryo transfer, goat.

• Abstract:

  The objective of this study was to evaluate the pregnancy rate in goats

  that had been inoculated with bisected embryos. We used 17 donors synchronized with

  an intravaginal device for 11 days and superovulated with 250UI of FSH-LH, starting on

  day nine, in six decreasing doses. On the last day of application, 100 μg of cloprostenol

  was administered. Detection of oestrus began soon after hormonal treatment, with the

  help of ruffians. Characterized the manifestation of estrus, the donors were covered. The

  recipients had the estrous cycle synchronized through intravaginal sponges, maintained

  for 11 days. On the ninth day 300 IU eCG and 75 μg cloprostenol were administered.

  Detection of estrus was performed by ruffians every 12 hours. Embryo collection

  was performed by uterine lavage using 200 ml of buffered phosphate buffered saline

  solution per donor, via transcervical, seven days after the coverage. 92 structures were

  recovered, but only 48 embryos were used (Grade 1 and 2). Of each three embryos

  obtained, one was bisected and the innovated halves in the same recipient. Whole

  embryos were innovated in pairs. Of the 16 embryo recipients with two whole embryos,

  nine (56.25%) presented pregnancy, three of them with double gestation (37.50% of

  embryos transferred). Of the 16 who received two hemi-embryos, four (25.00%) had

  single gestations and one (6.25%) double gestation (37.50%). Embryos bisected at a

  morula stage, regardless of quality, did not result in gestation; however, blastocysts

  of quality 1 obtained 100.00%. It was concluded that the innovation with two hemiembryos did not result in the improvement of the pregnancy rate in goats.

• Número de páginas: 15