SCAFFOLDS ROTOFIADOS DE POLICAPROLACTONA COM ÓLEOS DA AMAZÔNIA: OBTENÇÃO E ANÁLISE DE VIABILIDADE CELULAR
SCAFFOLDS ROTOFIADOS DE POLICAPROLACTONA COM ÓLEOS DA AMAZÔNIA: OBTENÇÃO E ANÁLISE DE VIABILIDADE CELULAR
DOI: https://doi.org/10.22533/at.ed.630112526023
Palavras-chave: açaí, biomateriais, jupati, PCL, PHBv, rotofiação, regeneração óssea
Keywords: açaí, biomaterials, jupati, PCL, PHBV, rotary-spinning, bone regeneration
Abstract: Bone fractures represent a growing problem, and using autogenous grafts is a limited alternative, which motivates the development of synthetic scaffolds to promote bone regeneration. In this context, adding vegetable oils to materials can aid in modulating inflammation, stimulating cell proliferation, and providing antioxidant and antimicrobial properties. Thus, this chapter addresses the development of electrospun scaffolds made of polycaprolactone (PCL) and polyhydroxybutyrate-co-valerate (PHBv) incorporated with Amazonian oils from açai (Euterpe oleracea) and jupati (Raphia taedigera), for potential use in bone tissue regeneration. The objective was to evaluate the morphology and cellular viability of the developed scaffolds using scanning electron microscopy and cytotoxicity analysis through the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assay. Additionally, the composition of the oils was analyzed by gas chromatography, revealing concentrations of over 68% of unsaturated fatty acids. PCL/PHBv blends with jupati oil exhibited cell viability greater than 98%, demonstrating excellent biocompatibility, whereas scaffolds composed solely of PCL showed reduced cell viability. Morphological analyses revealed a porous structure suitable for tissue regeneration. Overall, the results indicated that scaffolds containing jupati and açai oil hold potential for tissue engineering applications, suggesting further chemical, mechanical, and biological assessments.
- Atos Tavares Matos