RELATION BETWEEN PERIODONTAL CONDITION AND THE IN VITRO PRODUCTION OF HUMAN HSP60 INDUCED BY RECOMBINANT HmuY OF Porphyromonas gingivalis

• DOI: 10.22533/at.ed. 6901901108

• Palavras-chave: Atena

• Keywords: Periodontitis, HSP60, Porphyromonas gingivalis, host response.

• Abstract:

  Porphyromonas gingivalis is an

  important pathogen in chronic periodontitis

  whose virulence factors, such as HmuY, elicit

  the host response. The stress caused by the

  microbial challenge includes the production of

  chaperones responsible for cellular homeostasis

  and protein repair: the heat shock protein

  (HSP). The present study aimed to evaluate

  the production of HSP60 by cells of individuals

  with chronic periodontitis (CP) and without

  periodontitis (WP), under stimulus of HmuY.

  Eleven individuals with CP and twenty-six WP

  were examined using the following clinical

  periodontal parameters: probing depth (PD),

  bleeding on probing (BP) and clinical attachment

  level (CAL). Peripheral blood mononuclear cells

  (PBMC) were collected and cultured for 48

  hours with HmuY. The levels of human HSP60

  in the supernatant and cytosol were assessed

  through enzyme linked immunosorbent assay.

  No statistically significant differences were

  observed between the CP and WP individuals when the cells were cultured in the

  presence of HmuY (p= 0,261). However, a moderate positive correlation (r=0,666;

  p=0,025) was observed between the percentage of sites with CAL≥5 mm and the

  HSP60 levels induced by the recombinant protein HmuY in the PBMC. The highest

  levels of HSP60 were observed among the sites with the highest severity level of

  clinical attachment loss. The lipoprotein HmuY of P. gingivalis plays a role in the stress

  of the host cell.

• Número de páginas: 15