PURIFICATION OF A XYLANASE FROM Penicillium crustosum AND ITS POTENTIAL USE IN CLARIFYING FRUIT JUICE

• Palavras-chave: Atena

• Keywords: endoxylanase, fruit juice, filamentous fungi, Penicillium.

• Abstract:

  Xylan-degrading enzymes have

   

  been used in several industrial sectors, such

   

  as in pulp and paper processing, brewing,

   

  baking, animal feed, starch, textiles and wine

   

  production. This study aimed to purify an

   

  extracellular xylanase produced by Penicillium

   

  crustosum and investigate its potential for fruit

   

  juice clarification. The enzyme was purified

   

  after three chromatography columns (DEAE,

   

  CM-Sephadex, and Sephadex G-75), and it

   

  exhibited 23.4 kDa of molecular mass. The

   

  purified xylanase-I hydrolyzed the beechwood

   

  xylan and yield mainly xylobiose and xylotriose

   

  as end products. Furthermore, xylanase-I

   

  showed no cytotoxic effects and exhibited a

   

  significant increase in the clarification of mango,

   

  orange, strawberry, peach, apple, and papaya

   

  juices. This is also first report on the potential of

   

  a xylanase produced from Penicillium crustosum

   

  isolated from the Atlantic Forest that showed

   

  promising characteristics for biotechnological

   

  application.

• Número de páginas: 15