PRODUCTION OF ALKALINE PHOSPHATASE BY DENTAL PULP STEM CELLS IN INTERFACE WITH PLASMA MODIFIED TITANIUM

• DOI: 10.22533/at.ed.21920160116

• Palavras-chave: Palavras-chave

• Keywords: Alkaline phosphatase, Dental pulp, Plasma nitriding, Stem cells, Sputtering cage.

• Abstract:

  Introduction In this study a new method for plasma nitrided called discharge sputter cage was used for titanium surfaces to improve their biocompatibility with human tissue.Materials & Methods: The investigation of the biological response of this nitrided surface when in contact with cells with osteogenic potential is essential when you want to use this process in the manufacture of implants. Thus were evaluated in vitro the initial phenomena that culminate in osseointegration of implants, the main adhesion, proliferation and production of alkaline phosphatase from cells of the dental pulp focus. The physicochemical characterization of the surface of TiN was carried out by X-ray diffractometer, atomic force microscopy and wettability tests for determining the static contact angle. Results:Cell adhesion was enhanced by surface treatment plasma cathode cage being significantly greater adhesion on the surfaces nitridedTicp compared with the plate surface. Conclusions:Moreover, deposition of collagen fibers with production of alkaline phosphatase allows us to infer that there was a differentiation of cells of the dental pulp source in osteoblastic cells.

• Número de páginas: 20