pH e crescimento micelial de isolado de Pyricularia oryzae obtido de trigo
pH e crescimento micelial de isolado de Pyricularia oryzae obtido de trigo
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DOI: https://doi.org/10.22533/at.ed.141112613012
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Palavras-chave: meio de cultura, potenciais hidrogeniônicos, brusone do trigo, Magnaporthe oryzae
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Keywords: Culture médium, Hydrogen ion potential, wheat blast, Magnaporthe oryzae
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Abstract: pH is one of the main environmental factors that can affect the growth and development of phytopathogenic fungi in vitro. The objective of this study was to quantify the mycelial growth of P. oryzae, the causal agent of wheat blast, at different levels of hydrogen potential (pH). The fungus was transferred to potato-dextrose-agar (PDA, 39 g/L) culture medium, adjusted to pH 6, 7, 8, 9, 10, and 11; plates with pH 5.3 were used as control. After inoculation, the plates were kept in a growth chamber adjusted to a photoperiod of 12 hours of light and a temperature of 25 °C. After 10 days, colony diameter was measured in millimeters in both vertical and horizontal planes. The experiment was conducted in a completely randomized design, with 8 replicates per treatment. The data obtained were subjected to analysis of variance, and treatment means were compared using the Kruskal-Wallis test (P < 0.05) with the R software. Significant differences were observed among treatments. Mycelial growth ranged from 50.5 mm (control) to 66.8 mm (pH 11). The greatest growth occurred at alkaline pH values (11, 10, 9, and 8), which did not differ statistically from each other. The lowest growth occurred in the control (pH 5.3), pH 6, and pH 7 treatments, which also did not differ statistically. In general, fungi show optimal growth under acidic pH conditions; however, the results of this study demonstrate that P. oryzae also grows in alkaline culture media.
- Maria Fernanda Antunes da Cruz
- Victoria Dornelles Godinho
- Kauan Moraes Correa
- Taiane de Lima Zuse
- Taylan Alves Alderette