OTIMIZAÇÃO PARA OBTER UM EXTRATO RICO EM POLIFENÓIS DE FOLHAS DE MORINGA UTILIZANDO SONDA ULTRASSÔNICA
OTIMIZAÇÃO PARA OBTER UM EXTRATO RICO EM POLIFENÓIS DE FOLHAS DE MORINGA UTILIZANDO SONDA ULTRASSÔNICA
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DOI: https://doi.org/10.22533/at.ed.81324130610
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Palavras-chave: Alimentos saudáveis; Moringa oleífera; compostos antioxidantes; método de extração sustentável e emergente; compostos bioativos.
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Keywords: Healthy foods; Moringa oleifera; antioxidant compounds; sustainable and emerging extraction method; bioactive compounds.
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Abstract: Moringa has attracted the attention of researchers, being studied as a medicinal plant, abundant in vital nutrients, such as minerals, vitamins and proteins. It is rich in polyphenolic compounds, with anti-inflammatory, antibacterial and antioxidant activities. This study aimed to optimize conventional and ultrasound extraction methods, and evaluate the conditions of each extraction method, to better obtain polyphenolic compounds and antioxidant capacity of Moringa leaves. An experimental design 23 was carried out, evaluating the independent variables of the extraction methods (conventional and ultrasound) under the dependent variables (total phenolics, total flavonoids and antioxidant capacity). In relation to obtaining total phenolic compounds, flavonoids and antioxidant capacity, the method that obtained the best optimization response was the 130W ultrasonic probe, when compared to conventional extraction, by maceration. For total phenolic compounds, 670.86 ± 1.10 mg EAG g-1 were found, for total flavonoids it was 420.56 ± 1.30 mg EQ g1, and for antioxidant capacity the result obtained was 510.84 ± 1.40 µmol TEAC g-1 for the DPPH method, and for ORAC it was 510.69 ± 2.35 µmol Trolox g-1, through the ultrasonic probe, in just 10 min, 0.63 g of sample, at 50% amplitude. For extraction by maceration, total phenolic compounds were 324.55 ± 2.12 mg EAG g-1, for total flavonoids it was 188.12 ± 1.50 mg EQ g-1, and for antioxidant capacity the result obtained was 257.22 ± 1.45 µmol TEAC g-1 for the DPPH method, and for ORAC it was 288.20 ± 2.61 µmol Trolox g-1, in 15 min, 2g of sample at 70 ºC. It was proven that the 130 W probe was able to obtain high concentrations of polyphenolic compounds from moringa leaves, which are important for human health.
- Déborah Cristina Barcelos Flores
- Flávia Michelon Dalla Nora
- Caroline Pagnossim Boeira
- Naila Peil Marcuzzo
- Andressa Inês Schú
- Vanessa Ramos do Nascimento
- Cezar Augusto Bizzi
- Bruna Nichelle Lucas
- Anelise Pigatto Bissacotti
- Grazielle Castagna Cezimbra Weis