Organização do serviço de diagnóstico laboratorial do SARS-CoV2: a experiência do programa de vigilância da Covid-19 na UFPA.

• DOI: https://doi.org/10.22533/at.ed.909122430105

• Palavras-chave: Covid-19; SARS-Cov-2; RT-qPCR; diagnóstico.

• Keywords: Covid-19; SARS-Cov-2; RT-qPCR; diagnosis.

• Abstract: INTRODUCTION: Covid 19 is caused by SARS-Cov-19 retrovirus whose main symptoms as well as laboratory findings are common to other respiratory viruses. Definitive diagnosis is based on viral RNA or antigens identification in respiratory secretions and saliva samples. Antibodies identification in blood presents a peculiar pattern that does not guarantee active disease diagnosis. In March 2020, UFPA created the Covid-19 Surveillance Program at UFPA to assist workers at the critical moment of the pandemic when there were no well-defined standards for the flow of care for molecular diagnosis of Covid-19. AIMS: To propose an organization scheme for the SARS-CoV-2 laboratory diagnosis service using RT-qPCR in saliva samples in the clinical analysis laboratory and in the human and medical genetics laboratory at ICB/UFPA. MATERIAL AND METHODS: 1,313 asymptomatic workers at UFPA were attended, of which 1,153 performed the viral RNA test using the RT-qPCR method, 710 performed the serum IgG test and 586 performed both tests. Service flow was being organized according to the service demand by professionals involved in the project. UFPA Ombudsman carried out a survey with 50 users of the service, estimating the degree of satisfaction. RESULTS:98 (8.5%) subjects were positive in RT-qPCR tests and 161 (22.7%) were positive for IgG. We identified 35 (3.04%) inconclusive RT-qPCR results and 54 (7.6%) indeterminate IgG results.Correlation analysis, Odds Ratio and Relative Risk, revealed that there is a 3.5 times greater chance of RT-qPCR positive individuals also presenting positive IgG (p< 0.0001), which indicates IgG should not be specific for chronic condition of disease or these patients have already come into contact with virus and remained with a detectable titer at time of sample collect. The service was rated 9.02; its degree of importance was rated 9.55; satisfaction with the service reached a score of 7.84 and no user found it difficult to access the service.CONCLUSION: RT-qPCR performed on saliva samples were a practical and agile method. Results have shown IgG measurement is not exclusive to chronic cases. The service was very well evaluated by users, demonstrating the experience of the Covid-19 surveillance program at UFPA was successful in its proposal and execution.