OPTIMIZATION OF MICROWAVE -ASSISTED EXTRACTION OF ANTI-CANCEROUS CONSTITUENTS OF TURMERIC (Curcuma longa L.)BY RESPONSE SURFACE METHDOLOGY

• DOI: 10.22533/at.ed.5892224054

• Palavras-chave: MAE, Turmeric, Antioxidant, Anti Cancerous,

• Keywords: MAE, Turmeric, Antioxidant, Anti Cancerous

• Abstract:

  Turmeric (Curcuma longa L.) is a significant plant among cultivated spice crop in Pakistan.  It belongs to a ginger family (Zingiberaceae).Turmeric has been used as anti-oxidant and anti-cancerous and ant-inflammatory properties containing herb all over the world. The most active constituents of turmeric are curcumin and its derivatives demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC) collectively called curcuminoids. The present work reports on extraction of curcuminoids through Microwave-assisted technique optimized by Response Surface methodology (RSM).Three independent variables ethanol concentration (10-95%), extraction time (2-4minutes) , extraction temperature(250-400◦C) were selected. Total 27% yield of curcuminoids was calculated after crystallization of curcuminoids. The higher yield calculated at (95% ethanol), at time of (4minutes) and at (400◦C) temperature. The presence of curcuminoids was confirmed by UV-visible spectroscopy and curcumin showed maximum absorbance at 430nm to 465nm wavelength in ethanol solvent. Separation of curcuminoids was carried out by thin layer chromatography (TLC) analysis and results showed retention factor (RF) value at 0.78, 0.55, and 0.48 as curcumin, demethoxycurcumin, bis-demethoxycurcumin respectively. It is concluded that theoretical yield calculated by RSM was comparatively very close to experimental yield and Microwave-assisted extraction technique optimized by RSM was easiest and advanced way to obtain pure curcuminoids.

• Número de páginas: 10