The obtaining of substances

responsible to enhance health with the use of

plant extracts is proven to be healthier than those

formulated with the synthetized ones. Selective

conditions on the obtaining of natural extracts

with enhanced profile of phenolic constituents,

methylxanthines and antioxidant capacity was

investigated in this work using cold (CHE) and

hot hydroalcoholic extraction (HHE) of crude

guarana seeds. The solid waste seeds, obtained

from each extraction condition were evaluated in

terms of total phenolic content and antioxidant

capacity. Results indicate that highest recovery

of bioactive constituents in CHE was attributed

to the hydroalcoholic solution composed of

50% ethanol and to the seeds with 1.6 8mm.

Thin-layer chromatography detected positive

presence of phenolic constituents and caffeine,

which were confirmed quantitatively with HPLC.

Establishing comparison between CHM and

HHE, the highest profile of bioactive constituents

in the was obtained in HHE at 60oC. The use

of temperatures higher than 60oC resulted in

thermal degradation of phenolic constituents

and methylxanthines.