ÓLEO-RESINA DE COPAÍBA NÃO ALTERA OS NÍVEIS DE AMINOTRANFERASES E ESTEATOSE HEPÁTICA EM RATOS SAUDÁVEIS
ÓLEO-RESINA DE COPAÍBA NÃO ALTERA OS NÍVEIS DE AMINOTRANFERASES E ESTEATOSE HEPÁTICA EM RATOS SAUDÁVEIS
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DOI: 10.22533/at.ed.60023171016
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Palavras-chave: óleo-resina de copaíba, aminotransferases, esteatose hepática.
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Keywords: copaíba oleoresin, aminotransferases, hepatic steatosis.
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Abstract: With a vital role in metabolism, the liver is an organ that participates in various processes. It is known that copaiba oleoresin has beneficial effects on the liver tissue of animals with comorbidities; however, it is still understudied on normal rats. We aimed to evaluate copaiba oleoresin effects on aminotransferases levels, aspartate aminotransferase (AST), and alanine aminotransferase (ALT), as well as hepatic steatosis in healthy animals. Wistar rats were randomly divided to receive a standard diet (C, n=6) or a standard diet with copaiba oleoresin supplementation (C+O, n=6) at a dose of 200mg/kg/day for eight weeks. At the end of the experiment, body weight, fat weight, liver weight, hepatic tissue, and blood were collected for histological and biochemical analyses, respectively. Statistical analysis was performed using Student's t-tests or Mann-Whitney tests, with a significance level set at P<0.05. The study protocol was approved by the Ethics Committee for Animal Use at the Federal University of Mato Grosso (process # 23108.100444/2021-84). There was no difference in caloric intake and final body weight between the groups; however, the group supplemented with copaiba oil-resin showed lower visceral fat and consequently, a lower adiposity index. There was no difference in liver weight. Animals supplemented with copaiba oleoresin did not exhibit changes in liver injury markers, ALT and AST. Furthermore, there were no alterations in histological findings related to steatosis and inflammation. We conclude that copaiba oleoresin supplementation, 200 mg/kg/day, reduces body fat and does not affect hepatic aminotransferases levels or the microscopic liver morphology of animals without comorbidities, suggesting the safe use of this oleoresin.
- Gustavo Lima Guilherme
- Beatriz Alves Arrais de Morais
- Fernando Assis Brecho de Freitas
- Isabelle Lopes Silva
- Jéssica Cristine da Silva Garcia
- Luciana Ortega Telles
- Marcos Correa Dias
- Danilo Henrique Aguiar
- André Ferreira do Nascimento
- Gisele Facholi Bomfim
- Renata de Azevedo Melo Luvizotto