ISOLAMENTO, IDENTIFICAÇÃO E SELEÇÃO DE BACTERIAS PRODUTORAS DE α-AMILASE
ISOLAMENTO, IDENTIFICAÇÃO E SELEÇÃO DE BACTERIAS PRODUTORAS DE α-AMILASE
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DOI: https://doi.org/10.22533/at.ed.9142422032
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Palavras-chave: Fermentação submersa, Atividade enzimática, Enzimas.
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Keywords: Fermentation, Enzymatic activity, Enzymes.
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Abstract: Amylases are enzymes that hydrolyze starch molecules, releasing various products including dextrins and progressively small polymers composed of glucose units. It is of great importance in biotechnology with a wide field of applications, such as in the food industry, in breweries and fermented drinks, in the paper and cellulose industry, in the chemical and pharmaceutical industry, in the production of vitamins and antibiotics. This work aimed to isolate, characterize and select amylase-producing bacteria from samples from cassava, corn, moldy bread, cereals, fish feed, tapioca flour, bean grains, rice and soil. A total of twelve amylase-producing bacteria were isolated, these being a bacterium isolated from the corn root, later called Bacillus sp. MR1 presented the highest enzymatic activity index (4.06 cm) demonstrating the potential to produce the α-amylase enzyme.
- Cristian Jacques Bolner de Lima
- Monique Virães Barbosa dos Santos
- Marcos Benedito Santana