Intensidade de agitação na etapa de extração do fósforo do solo, com uso do extrator Mehlich-1
Intensidade de agitação na etapa de extração do fósforo do solo, com uso do extrator Mehlich-1
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DOI: https://doi.org/10.22533/at.ed.3802521115
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Palavras-chave: análise química; dessorção; oscilações por minuto; solução extratora.
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Keywords: chemical analysis; desorption; oscillations per minute; extracting solution.
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Abstract: The phosphorus (P) extraction method described by some authors does not specify the agitation intensity required when using the orbital shaker for obtaining the extract containing P, particularly when using the Mehlich-1 extractor. Thus, the aim of this study was to investigate the effect of different oscillation intensities (oscillations per minute, opm) on shaker adjustment during the extraction process, and to evaluate the impact of using a glass sphere during extraction. Samples from Chromic Acrisol (Clayic), representing the A, Bt1, and Bt2 horizons, were subjected to agitation at 100, 120, 140, 160, 180, 200, and 220 opm. In a second experiment, samples from the 0–20 cm layer of six representative soils from the Inland Tablelands of the Recôncavo Baiano were agitated at 100, 120, 140, 160, 180, and 200 opm on an orbital shaker. A third experiment involved agitation at 120, 180, and 220 opm, with and without the presence of a glass sphere. In all experiments, the samples were agitated for 5 minutes with 50 mL of Mehlich-1 solution, using three replicates per treatment. Calibration curves were generated using standard solutions containing 1, 2, 3, and 4 mg L-1 of P and 0.5, 1.0, 1.5, and 2.0 mg L-1, respectively. Absorbance readings were performed at 660 nm using a UV-VIS spectrophotometer. In all assays, the highest P concentrations in the extract were obtained at approximately 180 opm for the horizons and soils studied. Increasing agitation intensity beyond this point resulted in lower P concentrations in the extract, likely due to P re- adsorption onto the solid phase of the soil. Considering the average maximum points (PMx) from quadratic equations obtained for the studied horizons and soils, it can be concluded that 180 opm represents the optimal agitation intensity for maximizing P availability in the extract from soils with similar characteristics to those in this study. The use of the glass sphere did not result in higher phosphorus availability in the soil extract, regardless of the agitation intensity.
- Francisco de Souza Fadigas
- Oldair Del'arco Vinhas Costa
- Maria Amelia de Pinho Barbosa Hohlenwerger
- Magnison dos Santos
- Maria Paula Santa Ritta Amancio