IMOBILIZAÇÃO DE PEROXIDASE DE RAIZ FORTE POR APRISIONAMENTO E LIGAÇÃO COVALENTE EM SUPORTE HÍBRIDO DE ALGINATO-PECTINA E ALGINATO-PECTINA-AMIDO

• DOI: https://doi.org/10.22533/at.ed.9142422033

• Palavras-chave: Horseradish peroxidase. Armoracia rusticana. Biopolímero. Suporte híbrido.

• Keywords: Horseradish peroxidase. Armoracia rusticana. Biopolymer. Hybrid support.

• Abstract: Horseradish peroxidase (HRP) is an enzyme with excellent biocatalytic activity and potential industrial application. Aiming at large-scale applications, enzymatic immobilization emerges as an alternative for recovery and reuse of enzymes, enabling employability and reducing costs. In this sense, the use of biopolymers has been shown to be interesting, as they are non-toxic, biodegradable, renewable and highly available. However, there is still a lack of studies aimed at immobilizing HRP in hybrid biopolymers containing alginate, pectin and/or starch. Thus, the present study aimed to immobilize HRP, through entrapment and covalent binding, in beads of alginate-pectin and alginate-pectin-starch, in addition to a control (alginate). For this, different gel compositions were prepared and extruded in calcium chloride solution using a syringe and needle (Ø = 1 mm). The beads were cross-linked by inserting them in a 0.02% glutaraldehyde solution. The yield, efficiency and activity recovery parameters were evaluated at time 0 h (initial) and after seven days of storage. The immobilization of HRP in alginate-pectin-starch beads was superior to alginate-pectin when evaluating the set of parameters, resulting in an activity recovery of 11.21% at time 0 h and 10.51% after seven days of storage. However, it is important to highlight that the control formulation (alginate), among all, presented the best activity recovery after the storage time. Thus, in general, it can be concluded that the addition of pectin and/or starch in the composition of hybrid alginate gels has potential, however, further studies and optimizations are necessary.