Imobilização da enzima peroxidase em zeólita NaY por método sonoquímico

• DOI: https://doi.org/10.22533/at.ed.361112504044

• Palavras-chave: zeólita Y, peroxidase, imobilização

• Keywords: zeolite Y, peroxidase, immobilization

• Abstract: The synthesis of NaY zeolite was carried out using kaolin waste previously dried in an oven at 100 °C for 4 hours, followed by calcination at 700 °C for 2 hours. Approximately 2.1000 g of the calcined residue were used, mixed with Na₂SiO₃·5H₂O and 6 M NaOH solution, along with distilled water, placed in a PTFE cup in an autoclave at 90 °C for 20 hours. The product exhibited an octahedral bipyramidal shape, with intense peaks in the º2θ reflections at: 6.09º; 10.02º; 12.43º; 21.58º; 28.01º; and 33.28º, and a BET specific surface area of up to 100 m²/g. The protein concentrate was extracted from 0.996 kg of peach palm pulp (Bactris gasipaes), initially obtaining starch by centrifugation at 4 °C and 10,000 g for 5 minutes in the form of pellets. Subsequently, 250 mL of the supernatant were transferred to a centrifuge tube with the addition of 200 mL of a methanol:chloroform mixture (1:2), followed by centrifugation at 4 °C and 10,000 g for 7 minutes. The protein concentrate containing the enzymes was separated by filtration through a plastic sieve and stored between 4 °C and 10 °C in contact with a pH 4.5 buffer solution. Thus, an enzyme solution of 20 mg/mL was formed, and the activity of the peroxidase enzyme was verified using a 50 mM guaiacol solution and 10 mM H₂O₂. Immobilization was carried out by sonication at 40 W and 32 °C of 150 mg of support in contact with 600 μL of the 0.04 g/mL enzyme solution for 3 hours, 6 hours, and 9 hours. The absorbance of the control at 600 nm was measured using the Bradford reagent. The collection of the supernatant after 3 hours and 6 hours of sonication showed the highest values of enzyme loading on the unmodified and modified supports.