Genetic analysis and molecular phylogeny of zigzag leafhopper Maiestas dorsalis (Motschulsky) using mitochondrial COI gene

• DOI: https://doi.org/10.22533/at.ed.2572410078

• Palavras-chave: -

• Keywords: Auchenorrhyncha, mtCOI, DNA sequence, Samosir

• Abstract: The zigzag leafhopper Maiestas dorsalis (Hemiptera) is a tungro virus vector that cause damage on rice plants. Genetic analysis of M. dorsalis that be isolated from Samosir island, North Sumatra, Indonesia using partial DNA sequence of mitochondrial cytochrome oxidase subunit I (COI) DNA is still limited. This study aims to identify and to find out the genetic data of M. dorsalis that be derived from region of its mitochondrial cytochrome oxidase I gene (COI). Analysis was done by four steps, namely: Hopper collection on paddy field by using insect net, DNA extraction by using Zymo Tissue & Insect DNA Mini Preparation, amplification by PCR using My TaqTM HS Red Mix and DNA sequence analysis using ABI PRISM 3730xl Genetic Analyzer. Primer cocktail tRWF-Mlep was used in DNA amplification step. The research result pointed out that COI DNA fragment of M. dorsalis has length 521 bp. This COI DNA sequence was dominated by A and T(U) bases with concentration 74.30%. The concentration of T(U), C, A and G nucleotides in the COI sequence were 35.90%, 13.40%, 38.44%, and 12.30%, respectively. Identification of M. dorsalis based on this COI DNA sequence confirmed the identification result based on its morphological characters.