Evaluation of Heterologous Protein Expression at Different Concentrations of MgSO4 and IPTG in Escherichia coli W110

• DOI: 10.22533/at.ed.21419210610

• Palavras-chave: Atena Editora

• Keywords: Escherichia coli, Heterologous protein, H-ras.

• Abstract:

  Since the improvement of gene sequencing techniques, the expression of heterologous proteins is gaining increasing relevance given the possibility of large-scale production when compared to the isolation of these same molecules in their original sources. However, heterologous expression systems continue to require for a suitable induction relevant amounts of synthetic molecules like IPTG (Isopropyl β-D-1-thiogalactopyranoside) which have toxicity and cost limitation when used in large scale. Thus, the objective of this work was to evaluate the effects of bivalent salts such as MgSO4 in association with IPTG on heterologous expression system pDMI.1 for a possible better cost-benefit proposal in the current heterologous protein expression protocol. Magnesium Sulfate contributes to an increase in bacterial physiology as a whole, inducing a higher protein expression acting as an indirect and alternative inducer in these conditions, with the advantages of presenting low cost and low cellular toxicity. Based on these obtained data, we propose the usage of low MgSO4 molar concentrations in current heterologous protein expression protocols performed in our group for the pDMI.1 vector. Additional studies are still needed in order to evaluate the effects of this salt on different expression systems at wide range of microorganisms.

• Número de páginas: 15