ESTUDO DAS ALTERAÇÕES INFLAMATÓRIAS E MORFOLÓGICAS PULMONARES EM MODELO ANIMAL DE PNEUMOPERITÔNIO

• Palavras-chave: Palavras-chave

• Keywords: Laparoscopy; Experimental; Pneumoperitoneum; Cytokines; Oxidative Stress; Lung Injury

• Abstract:

  Background: Laparoscopic surgeries require pneumoperitoneum achieved by pressure-controlled insufflation with carbon dioxide into the peritoneal cavity. This condition changes the respiratory metabolism and promotes lung damage.

  Purpose: The aim of the present study was to compare the effect of different levels of intra-abdominal pressure (IAP) in a ventilated rat model with normal lungs.

  Methods: Forty-eight Wistar rats were selected at random and eight rats were assigned to each of following groups. The Sham group was subjected to a sham operation without pneumoperitoneum, the other groups were subjected to CO₂ pneumoperitoneum with 5, 8, 10, 12 or 14 mmHg intra-abdominal pressure for 60 minutes. All animals were mechanically ventilated. At the end of the experiment, the animals were euthanatized and had their lungs removed for analysis. Lipid peroxidation, myeloperoxidase activity, measurements of cytokines, and histopathological analysis were performed.

  Results: In the IAP5 group, all analyses were lower compared with those of other groups. TNF-alpha, IL-1 beta, IL-6, lipid peroxidation and myeloperoxidase activity were higher in groups IAP10, IAP12, and IAP14 compared with those of groups IAP5 and IAP8. These results were supported by histopathological examination.

  Conclusions: These findings suggest that high pressure increase oxidative stress and inflammatory induced lung damage after pneumoperitoneum.

• Número de páginas: 12