Estudio Molecular Comparativo de la Frecuencia de Dengue y Zika en Suero y Saliva de Mujeres en Edad Reproductiva del Estado de Guerrero
Estudio Molecular Comparativo de la Frecuencia de Dengue y Zika en Suero y Saliva de Mujeres en Edad Reproductiva del Estado de Guerrero
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DOI: https://doi.org/10.22533/at.ed.8022401044
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Palavras-chave: Dengue, Zika, Suero, Saliva, qRT-PCR
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Keywords: Dengue, Zika, Serum, Saliva, RT-qPCR
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Abstract: Introduction. The Zika virus causes neurological complications and fetal death. Like Dengue, they are associated with spontaneous abortions. We analyzed the frequency of these viruses in serum and saliva of Guerrero women of reproductive age. Methodology. 30 women with febrile reaction were included. RNA was extracted by Trizol and Real-time quantitative polymerase chain reaction (RT-qPCR). Results. 66.7% presented Dengue virus in serum and saliva. We detected Zika virus RNA in 63.3% (saliva) and 50% (serum). We found that more than 50% of the women had viral coinfection. The frequent symptoms were fever >38°C, headache, myalgia, arthralgia, retro-ocular pain and conjunctivitis. Conclusions. The frequency of Dengue and Zika viruses by RT-qPCR in serum and saliva in the febrile period represents a problem in reproductive health and evidence of saliva as a non-invasive and easily obtained alternative for the timely detection of these viruses in women of age. fertile.
- Luz del Carmen Alarcón Romero
- Ma. Isabel Zubillaga Guerrero
- Carlos Ignacio Machain Williams
- Berenice Illades Aguiar
- Ana María Espinosa García
- María José Montenegro Rivera
- Julio Alberto Pinzón Barrera
- Yazmín Gómez Gómez
- Mariana Villegas Hernández