Effect of temperature and salinity on the production of carotenoids and lipids by marine microalga
Esta pesquisa identificou os carotenoides presentes no extrato de Dunaliella tertiolecta e avaliou os efeitos da temperatura e da salinidade do meio na composição elementar e nos teores de lipídios e carotenoides desta microalga marinha cultivada em um fotobiorreator airlift. Os experimentos foram realizados de acordo com um delineamento composto central rotacional (DCCR). Seis carotenoides foram identificados por cromatografia líquida de alta eficiência (CLAE): trans-luteína, trans-βcaroteno, trans-zeaxantina, trans-neoxantina, trans-anteraxantina e 9-cis-β-caroteno. As condições ideais para a produção de carotenoides e lipídios por D. tertiolecta foram de 27 a 29 ° C e 0,7 a 0,8 M de NaCl. Nas condições testadas, as maiores quantidades de lipídios, luteína, β-caroteno e zeaxantina obtidas foram, respectivamente, 11,75 ± 1,8 %, 1315 ± 36 µg g-1, 732 ± 51 µg g-1 and 244 ± 76 µg g-1.
Effect of temperature and salinity on the production of carotenoids and lipids by marine microalga
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Palavras-chave: Dunaliella tertiolecta, airlift, carotenoides, lipídios.
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Keywords: Dunaliella tertiolecta, airlift, carotenoid, lipids.
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Abstract:
This research identified the carotenoids present in Dunaliella tertiolecta extract and evaluated the effects of temperature and medium salinity on the elemental composition and lipid and carotenoid contents of this marine microalga cultured in a flat-panel airlift photobioreactor. The experiments were performed according to a central composite design (CCD). Six carotenoids were identified by high-performance liquid chromatography (HPLC): all-trans-lutein, all-trans-β-carotene, all-trans-zeaxanthin, all-trans-neoxanthin, all trans-antheraxanthin and 9-cis-β-carotene. The ideal conditions for carotenoid and lipid production by D. tertiolecta were 27 to 29 °C and 0.7 to 0.8 M NaCl. Under test conditions, the greatest amounts of lipid, lutein, β-carotene and zeaxanthin obtained were, respectively, 11.75 ± 1.8 %, 1315 ± 36 µg g-1, 732 ± 51 µg g-1 and 244 ± 76 µg g-1.
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Número de páginas: 15
- Nicéia Chies Da Fré
