EFEITOS DA MELATONINA NA HISTOMORFOMETRIA DOS RINS E DO FÍGADO DE RATOS WISTAR EXERCITADOS COM DOENÇA DE PARKINSON
EFEITOS DA MELATONINA NA HISTOMORFOMETRIA DOS RINS E DO FÍGADO DE RATOS WISTAR EXERCITADOS COM DOENÇA DE PARKINSON
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DOI: https://doi.org/10.22533/at.ed.0051125180311
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Palavras-chave: Doença de Parkinson. Exercício. Melatonina. Figado. Rins.
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Keywords: Parkinson's disease. Exercise. Melatonin. Liver. Kidneys.
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Abstract: Parkinson's disease (PD), the second most common neurodegenerative condition of the central nervous system (CNS), is an idiopathic disorder characterized by systemic motor and non-motor manifestations. The overall objective of this study was to analyze the effects of melatonin on the kidneys and livers of exercised rats with Parkinson's disease. A total of 64 male Wistar rats (Rattus norvegicus, var. albinus) weighing between 250 and 450 g and aged 40 days were used. The animals received melatonin treatment via intraperitoneal injections at a dose of 20 mg/kg, 5 days a week for 5 weeks prior to PD induction surgery. To assess motor function, all animals underwent motor testing after PD induction surgery. The parallel bar, false step, and open field tests were used. The progressive resistance exercise protocol was performed 5 times a week for 4 weeks, for 30-35 minutes daily. For PD induction, 6-OHDA (Sigma-Aldrich Brasil Ltda) was applied at a dose of 2 µL/10 µg dissolved in 0.02% ascorbic acid in the striatum at the target coordinates for the striatum: AP = 4.4, LR = -1.2, and DV = -7.8 from bregma. The kidneys and liver were removed for histological processing and stained with hematoxylin and eosin (HE). The capsule area was calculated by subtracting the glomerular tuft area and glomeruli counted in the kidneys. In the liver, the number of hepatocytes was measured, calculating the theoretical diameter. It is concluded that melatonin associated with progressive resistance exercise (PRE) was able to reduce the area of Bowman's space in diseased rats. Furthermore, the application of these two factors, in isolation, was able to reverse the decrease in glomerular tuft diameter caused by PD. Regarding the liver, melatonin, in some cases combined with physical exercise, promoted a decrease in the area, perimeter, and theoretical diameter of hepatocytes.
- Kananda Oliveira Garcia Ruiz
- José Victor Ribeiro Silva Gomes
- Ismael Augusto Lima Santos
- Gustavo Amorim Alves
- Laila Cristina Moreira Damázio