Efeito da quatidade da mistura e da filtração na disponibilidade de fósforo (P) no extrato de solo obtido com Mehlich-1
Efeito da quatidade da mistura e da filtração na disponibilidade de fósforo (P) no extrato de solo obtido com Mehlich-1
-
DOI: https://doi.org/10.22533/at.ed.3802521111
-
Palavras-chave: teor de fósforo; extração de fósforo; decantação; agitação.
-
Keywords: phosphorus content; phosphorus extraction; decantation; shaking.
-
Abstract: Several methods for phosphorus (P) extraction have been developed and tested under various conditions in search of those that yield P levels equivalent to the amounts taken up by cultivated plants. One example is the Mehlich-1 extractant solution, which has been modified since its original formulation proposed by Mehlich (1953). This study aimed to evaluate the effect of different volumes of extractant solution and soil, agitated in 125 mL and 100 mL flasks, as well as the use of extract filtration. Soil samples were shaken at 180 rpm for 5 minutes and then allowed to settle for 16 hours, with three replicates. The treatments were: 10 mL soil:50 mL Mehlich-1 solution (in 125 mL Erlenmeyer flasks); 5 mL soil:50 mL Mehlich-1 solution (in 125 mL Erlenmeyer flasks); and 2.5 mL soil:50 mL extractant solution (in 100 mL Erlenmeyer flasks), each combined with either filtration or decantation of the extract. Absorbance was measured at 660 nm in a molecular absorption spectrophotometer using the molybdenum blue method. P concentrations in the extract, measured immediately after shaking with filtration, were significantly lower than those obtained after decantation (16 h), without filtration. Significant effects of soil and extractant volumes were observed, with P concentrations from the 10 mL soil:50 mL Mehlich-1 mixture being significantly lower than those from the other treatments. Differences in P levels were also found among the soils studied. The results indicate that, for extracting only P or for preparing extracts intended for multielement analysis by techniques such as ICP-OES, the volumes of soil and Mehlich-1 extractant can be reduced.
- Francisco de Souza Fadigas
- Sandra Maria Conceição Pinheiro
- Ivonete Oliveira de Jesus