EFEITO ANTIOXIDANTE DOS FRUTOS DE Schinus terebinthifolius Raddi SOBRE O ESTRESSE OXIDATIVO EM ERITRÓCITOS HUMANOS
EFEITO ANTIOXIDANTE DOS FRUTOS DE Schinus terebinthifolius Raddi SOBRE O ESTRESSE OXIDATIVO EM ERITRÓCITOS HUMANOS
-
DOI: https://doi.org/10.22533/at.ed.5122405041
-
Palavras-chave: bioprospecção, antioxidante, plantas medicinais, pimenta-rosa, Cerrado
-
Keywords: bioprospecting, antioxidant, medicinal plants, pink pepper, Cerrado
-
Abstract: Oxidative stress is associated with the onset and progression of several diseases, amongst them cancer, diabetes, cardiovascular and neurodegenerative diseases. The treatment of these diseases involves drugs which, in many cases, cause excessive costs, side effects and a reduction in the life quality of the affected individuals. Therefore, the bioprospecting of medicinal plants with antioxidant potential has become a relevant alternative for oxidative stress control and, consequently, associated chronic diseases. Schinus terebinthifolius Raddi is a native species of the Brazilian Cerrado, used in folk medicine for various finality. The fruits of S. terebinthifolius, known as pink pepper, are widely used as a condiment in world gastronomy. In this context, this study investigated the chemical composition and antioxidant potential of the methanolic extract of S. terebinthifolius (EMF-St) fruits. The chemical composition of EMF-St was evaluated by different colorimetric methods for the quantification of ascorbic acid, β-carotene, lycopene, phenolic compounds and condensed tannins. The antioxidant activity of EMF-St was researched by the DPPH• and ABTS•⁺ free radical scavenging methods. The effect of EMF-St against oxidative stress has been evaluated in human erythrocytes induced with AAPH. The analysis of the chemical composition of EMF-St revealed the majority presence of phenolic compounds. In the DPPH• and ABTS•⁺ assays, the EMF-St IC50 was 14.58 ± 2.16 and 9.96 ± 1.04 μg/mL, respectively. In human erythrocytes, EMF-St did not cause hemolysis up to a concentration of 100 μg/mL, as did ascorbic acid. Under oxidative stress conditions induced by AAPH, human erythrocytes treated with EMF-St showed a 54% reduction in the content of malondialdehyde generated at a concentration of 75 µg/mL. Taken together, the results indicate that the methanolic extract of S. terebinthifolius fruits has the potential to reduce oxidative stress, opening perspectives for the development of a new biotechnological product for the prevention and/or treatment of diseases associated.
- Maria Victória Benites Rodrigues
- Stefany Cardoso Brandão
- Emilha Uzum Papaya
- Iara Souza Santos
- Sarah Lam Orué
- Isamara Carvalho Ferreira
- Daniel Ferreira Leite
- Paola dos Santos da Rocha
- Jaqueline Ferreira Campos
- Edson Lucas dos Santos
- Kely de Picoli Souza