DOUBLE MINUTE CHROMOSOMES in Anastrepha fraterculus (Wiedemann) (DIPTERA: Tephritidae): a model for cancer studies

• DOI: 10.22533/at.ed.97523250912

• Palavras-chave: -

• Keywords: cytological markers, environmental contamination, stress,

• Abstract:

  Anastrepha fraterculus (Wied.), is known as the South American Fruit
  Fly. In order to develop efficient control strategies, we need deep knowledge on its
  biology along with periodical studies on population dynamics. Citogenetic studies on
  natural populations of this pest fly made it possible to reveal the presence of double
  minute chromosomes (DMs) in several natural populations of this insect. Cytogenetic
  studies performed in our insect laboratory, allowed the genetic analysis through a
  genealogical methodology. The establishment of families made it possible the genetic
  studies allowing the rigorous identification, characterization and confirmation of new
  chromosomal variants, frequently misinterpreted when the materials analyzed only
  come from nature. Double minute chromosomes -considered a type of chromosomal
  rearrangement- are extra chromosomal gene copies. This study sought to answer:
  What role do these DMs chromosome play in laboratory populations of the pest derived
  from natural populations? How are they transmitted from one generation to another?
  To answer our questions, we analyzed, for 25 generations, two laboratory populations
  derived from Tucuman (T) and Buenos Aires (BA -tester) guava,. Data were recovered
  from cytological analysis of ganglia preparations revealed with H33258. The natural
  population from BA didn’t carry DMs. Results showed DMs in flies of both laboratory
  populations. DMs were transmitted from parents to progenies through 25 generations,
  their transmission was randomized in number. DMs are the cytological expression of
  resistance mechanisms used by the pest as a response to environmental stress. DMs
  in BA strain marked the change to the laboratory environment. A. fraterculus is a model
  insect for the study of cancer.