Corynebacterium pseudotuberculosis protein extract induces IP10 production in blood samples of individuals with pulmonary tuberculosis

• DOI: 10.22533/at.ed.4352001075

• Palavras-chave: CORYNEBACTERIUM PSEUDOTUBERCULOSIS; CYTOKINES; MYCOBACTERIUM TUBERCULOSIS; TUBERCULOSIS; ANTIGENICITY

• Keywords: CORYNEBACTERIUM PSEUDOTUBERCULOSIS; CYTOKINES; MYCOBACTERIUM TUBERCULOSIS; TUBERCULOSIS; ANTIGENICITY

• Abstract:

  Mycobacterium tuberculosis and Corynebacterium pseudotuberculosis are part of the CMRN group and share similarities among some virulence/pathogenicity factors. The objective of the present study was to use semi-purified extracts of C. pseudotuberculosis strains to evaluate antigenicity in samples of individuals diagnosed with active pulmonary tuberculosis. Voluntary participants with  positive tuberculosis (TB) diagnosis and treatment-naïve tuberculosis (n=28), without complaints, no history or symptomatology of TB or M. tuberculosis infection (n=28). With heparinized blood, cell stimulation was carried out using mitogen (PWM) and C. pseudotuberculosis protein extract (PAT-10, VD57 and FRC-41 strains). For cytokines measurement, BD Cytometric Bead Array (CBA) was used. Semi-purified extracts of all three C. pseudotuberculosis strains induced production of cytokines in blood samples from studied groups. In addition, they differentiated studied groups when compared to IFN-γ and IP-10. C. pseudotuberculosis strains extracts PAT10, VD57 and FRC41 induced production of IFN-γ, IP-10 in blood samples from individuals infected with M. tuberculosis. Under stimuli of extracts used, the detection of IFN-γ and IP-10 differentiated  the control group and active TB groups.

• Número de páginas: 12