COMPARAÇÃO ESTRUTURAL DA ALENO ÓXIDO CICLASE (AOC) EM TRÊS ESPÉCIES VEGETAIS: TOMATEIRO, MILHO E ARABIDOPSIS
COMPARAÇÃO ESTRUTURAL DA ALENO ÓXIDO CICLASE (AOC) EM TRÊS ESPÉCIES VEGETAIS: TOMATEIRO, MILHO E ARABIDOPSIS
-
DOI: https://doi.org/10.22533/at.ed.6882409114
-
Palavras-chave: Bioinformática; defesa de plantas; bioquímica.
-
Keywords: Bioinformatics; plant defense; biochemistry.
-
Abstract: Jasmonic acid is one of the main plant defense hormones and relies on the interaction of a series of enzymes to trigger the appropriate response. Allene Oxide Cyclase catalyzes the production of the last plastidial intermediate (12-Oxophytodienoic Acid). Thus, the aim of this work is to perform a structural comparison of these proteins in two agricultural species (tomato and maize) and a model organism (Arabidopsis). For this purpose, the primary sequence was collected from two genetic data platforms (NCBI and UniProt). Then, these data were processed using the following software: Clustal Omega, SwissModel, and EXpasy ProtParam. The following results were obtained: The amino acid sequences of AOCs from Arabidopsis, Tomato, and Maize are highly conserved, preserving functional units through evolution; The non-essential portion of the amino acid sequences appears to be subject to genetic drift, not reflecting the evolutionary function of the protein in the plant defense system; The essential part of the AOC, crucial for catalysis and regulation of reactions, is evolutionarily conserved due to its importance in the plant defense system; alterations in physicochemical properties can affect the catalytic ability of AOC because of steric hindrance or similar charge repulsion; the variation in the number of amino acids and molecular weight among the AOCs of the three species suggests that some amino acids do not have a specific function; and the AOC is sensitive to extreme temperature and pH conditions, which can compromise its function and the defense mechanisms of plants in the three species investigated.
- Mônica Trindade de Abreu
- Carlos Augusto Cavalcante de Oliveira