CLADISTIC ANALISYS IN GEMINIVIRIDAE: AN EVIDENCE OF MULTISPECIFICITY FOR CULTIVARS HOSTS

• DOI: 10.22533/at.ed.1381912025

• Palavras-chave: atena

• Keywords: Maximum Likelihood; Mosaic virus; Tomato.

• Abstract:

  In recent years the Geminiviridae

  family virus has been intensively studied

  due the severity of the diseases caused in

  several cultures of economic importance; like

  bean, cotton, corn, tomato and cassava. The

  objective of this work was to do a cladistic

  inference of Begomovirus populations through

  computational tools. Viral genome sequences

  were obtained from NCBI (https://www.ncbi.nlm.

  nih.gov/), totaling 297 sequences from different

  countries. The sequences were aligned with the

  Bioedit program, using the ClustalW algorithm.

  After the alignment, the cladogram was obtained

  through the Maximum Likelihood method in the

  MEGA program, with bootstrap analysis of 1000

  replicates. ModelTest was used to select the

  most suitable evolutionary model (Reversible G

  + I) for the data set. The results showed that

  the bootstrap values in the clades ranged from

  9 to 100. Pepper, potato and watermelon virus

  sequences were used with outgroup, but they

  were grouped into clades shared by the viruses

  obtained in the Tomato strain, revealing an

  evidence that the mosaic virus in these cultivars

  belong to the same species and same genus.

• Número de páginas: 15