BIOTECHNOLOGICAL PRODUCTION OF ENZYMATIC EXTRACT WITH CELULOLYTICAL ACTIVITY FROM AGROINDUSTRY RESIDUES

• Palavras-chave: Atena Editora

• Keywords: Filamentous Fungi, Fermentation, Cellulolytical Activity

• Abstract:

  Beer consumption in Brazil has generated a great environmental problem due to the final destination of the malt bagasse. To minimize generation of this residue implies in the use of this bagasse in biotechnological researches of industrial interest, as production of enzymes, whose characteristics are their high specificity in catalytic action, high efficiency in moderate temperature, pressure and pH conditions and minimization of toxicological and environmental problems. This work aims to comparatively evaluate the production of exoglucanase among the filamentous fungi Aspergillus niger and Penicillium glabus from the malt bagasse moistened in semi-solid fermentation medium and water used to wash malt bagasse as substrate of submerged fermentation. In order to evaluate the production of exoglucanases, was produced a fermentation system of malt bagasse washed and moistened with water, a spore suspension with 7 days growth and spore numbers equal to 5x107 spores / mL. Also, water used to wash crude malt bagasse was used as substrate to submerged fermentation of extracts with celulolytical activity, using same filamentous fungi. It is observed na interesting behavior of P. glabus in the washing water (0,32 μmol/min), obtaining the best production of cellulolytic activity in relation to the other conditions of fermentation.

• Número de páginas: 15