BENEFÍCIOS DA TÉCNICA DE INJEÇÃO INTRACITOPLASMÁTICA DE ESPERMATOZOIDES NA REPRODUÇÃO DE CAVALOS
BENEFÍCIOS DA TÉCNICA DE INJEÇÃO INTRACITOPLASMÁTICA DE ESPERMATOZOIDES NA REPRODUÇÃO DE CAVALOS
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DOI: https://doi.org/10.22533/at.ed.441122601049
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Palavras-chave: ICSI. Reprodução. Embriões equinos.
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Keywords: ICSI. Reproduction. Equine embryos.
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Abstract: Intracytoplasmic Sperm Injection (ICSI), initially developed for the treatment of human infertility, has been incorporated into veterinary medicine as an important reproductive biotechnology, particularly in equine reproduction. The technique consists of the injection of a single spermatozoon into the cytoplasm of an oocyte previously matured in vitro, generally obtained through transvaginal ultrasound-guided follicular aspiration. This study aimed to review the main benefits of ICSI in equine reproduction. A literature review was conducted using national and international electronic databases, emphasizing publications from the last 20 years and employing descriptors related to the technique and equine reproductive physiology. ICSI is especially indicated in cases of subfertility, poor semen quality, or limited availability of high genetic value semen, enabling more efficient use of genetic material. The procedure involves oocyte recovery and in vitro maturation, injection of a single spermatozoon — fresh or cryopreserved — and subsequent oocyte activation, often assisted by piezoelectric pulses. Scientific evidence demonstrates high effectiveness of the technique in embryo production and the propagation of superior genetic material, consolidating its application in contemporary equine reproduction.
- Lucas de Melo Lôbo
- Rógenes Ferreira Caetano
- Valesca Barreto Luz