AVALIAÇÃO ESTRUTURAL DE ESPERMATOZOIDES CAPRINOS LOCALMENTE ADAPTADOS CRIOPRESERVADOS NO PERÍODO SECO

• DOI: 10.22533/at.ed.2631912098

• Palavras-chave: sêmen, ultraestrutura espermática, banco de germoplasma.

• Keywords: semen, spermatic ultrastructure, germplasm bank.

• Abstract:

  This work aimed to evaluate the effect of the time of year on the structural

  integrity of locally adapted goat spermatozoa. Eighteen adult goat breeds, aged

  between 2 and 5 years old, from the Blue (AZ), Canindé (CD) and Moxotó (MX) breeds

  were used. The semen collections were performed in the morning, using the artificial

  vagina method, with the aid of an estrogenized female. After collection, a pool of

  ejaculates of the same race were formed for analysis, selecting the ejaculates suitable

  for cryopreservation. The semen was diluted in Tris-Gemma medium and frozen by

  the automated method and stored in a cryogenic cylinder at -196ºC. After thawing,

  spermatozoa were analyzed using fluorescent probes, determining plasma membrane

  integrity, acrosomal integrity and mitochondrial activity of sperm cells at different postthaw times (0, 60, 120 and 180 minutes). In the structural analysis, a high percentage of

  cells with plasma membrane integrity (≥50%) was observed, without differing between

  post-thaw times. The AZ breed showed low mitochondrial activity indexes (37.65%),

  which may have had a direct effect on sperm motility (66%) in fresh semen during this

  period of the year. The acrosome integrity of the sperm cells were high in all races

  evaluated in this period (≥50%), and there was no difference even after 3 hours of

  thawing of the samples. It is concluded that the cryopreservation of the semen of these

  breeds during the dry period can be used as a tool for the genetic conservation of these

  ecotypes, as it maintains acceptable seminal andrological standards.

   

• Número de páginas: 15