AVALIAÇÃO DE ATIVIDADE UREASE PARA OBTENÇÃO DE UM PRODUTO BIOCIMENTANTE PARA A CONSTRUÇÃO CIVIL A PARTIR DA SOJA
AVALIAÇÃO DE ATIVIDADE UREASE PARA OBTENÇÃO DE UM PRODUTO BIOCIMENTANTE PARA A CONSTRUÇÃO CIVIL A PARTIR DA SOJA
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DOI: https://doi.org/10.22533/at.ed.384132410127
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Palavras-chave: enzima urease, Glycine Max L., precipitação de carbonato de cálcio, biocimento sustentável.
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Keywords: enzyme urease, Glycine Max L., calcium carbonate precipitation, sustainable biocement.
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Abstract: The precipitation of calcium carbonate by urease is a technology of low environmental impact that has been gaining attention in the engineering area in recent decades, being used bacteria or plants to obtain a biocement product for geotechnics or in the manufacture of sustainable materials as a substitute for non-renewable binders. The enzyme urease, present in soybeans (Glycine Max), catalyzes the hydrolysis of urea, leading to the production of calcium carbonate, which acts as a binder for soil particles or other inert material. Several studies have used soybean for enzymatic extraction, however, the best way to obtain soybean extract is still not clear in the literature, from this, the objective was to evaluate the urease activity from soybeans with and without ethanol. A soybean extract (SE) was obtained with distilled water and another with 20% ethanol (ESE), both with 100g of soybean per liter. The urease activity (UA), pH and electrical conductivity of the extracts were evaluated in the presence of different concentrations of urea. The results showed that the AU of the extracts was 25.72 and 21.69 mM/min for SE and ESE, respectively. Values above those found in the literature, however, ethanol has not been shown to improve enzymatic activity. The pH showed a typical change and a stabilization trend, confirming the urease activity of both extracts, and the electrical conductivity confirmed that the ethanol did not improve the performance of the solution. The concentration of urea that obtained the highest electrical conductivity was 0.2 mol/L for SE. Urease activity evaluations are preliminary studies of great importance for the feasibility of obtaining a sustainable cement product for application in engineering.
- Luiza Beatriz Gamboa Araújo Morselli
- Lara Alves Gullo Do Carmo
- Jessica Torres dos Santos
- Gustavo Luís Calegaro
- Talisson Natan Tochtenhagen
- Estevan Alcântara Huckembeck
- Rafael De Lima Rodrigues Chiquine
- Bruno Nunes Hubner
- Marcos Antonio da Silva
- Leonardo Bubolz Leal
- Julia Kaiane Prates Da Silva
- Robson Andreazza