ATIVIDADE DE CELULASES, BETA-GLICOSIDASES E XILANASES DE Trichoderma harzianum E Trichoderma asperellum EM BAGAÇO DE CANA DE AÇÚCAR

• Palavras-chave: Celulase, xilanase, b-glicosidase, bagaço de cana de açúcar, Trichoderma.

• Keywords: Cellulase, xylanase, b-glucosidase, sugarcane bagasse, Trichoderma.

• Abstract:

  Brazil is the largest producer of

  sugar cane, generating about 147 million tons of

  sugarcane bagasse per year. This residue could

  be a important for the production of cellulolytic

  and hemicellulolytic enzymes that have several

  industrial applications, as in the production

  of biofuels. This present study evaluated the

  production of cellulases, b-glycosidases and

  xylanases by Trichoderma species isolated in the region of Londrina-PR and grown

  in sugarcane bagasse (SB). Solid state fermentation (SSF) was performed with SB

  from local commercial sugarcane (SBC) and industry sugarcane bagasse (SBI).

  Cultures grown in 50 mL Erlenmeyers containing 1.4 g of BC were moistened with

  10 mL of Vogel’s solution. The inoculum of Trichoderma harzianum Rifai (Thh1) and

  Trichoderma asperellum (Tha2 and Tha3) consisted of 400 μl of 0.1% (v / v) tween

  80 solution containing 1.108 UFC mL-1, incubated at 28 ± 2 ° C for seven days. Thh1

  was the best producer of cellulases (with) 8.38 U/g in SBI and 6.46 U/g in SBC and

  xylanases 0.37 U/g among the evaluated fungi, the species T. asperellum, Tha2 and

  Tha3, also produced cellulases preferentially 4.17 U/g than xylanases 0.15 U/g for

  both substrates. The activity of b-glycosidases for Tha2 and Tha3 was higher when

  compared with the result found for Thh1, 0.21 U/g and 0.13 U/g, respectively. The

  results show that the fungi preferentially produced cellulases in SBI, suggesting their

  potential application to the use of this residue so abundant in Brazil.

• Número de páginas: 15