"ANTIMICROBIAL ACTIVITY OF SILVER NANOPARTICLES PRODUCED BY GREEN SYNTHESIS FROM ENDOPHYTIC FUNGI PENICILLIUM SPP

• DOI: https://doi.org/10.22533/at.ed.2922415082

• Palavras-chave: -

• Keywords: Penicillium spp., endophytes, green synthesis, metallic nanoparticles, AgNO3, microbial inhibition

• Abstract: The increase in bacterial resistance to antimicrobials has become a major problem for public health in recent decades. Endophytic fungi, that lives in mutualistic association with plants, are a rich source of bioprospecting for such bioactive compounds. The objective of the present project was to synthesize silver nanoparticles through green synthesis, an ecologically sustainable approach that uses biological organisms as biofactories to the production of metallic nanoparticles. The utilization of metallic nanoparticles can be applied in the study of the control of resistance to traditional antimicrobials using inhibition as prevention of microbial diseases. The endophytic filamentous fungi Penicillium spp., isolated from Polygala paniculata a plant collected in Brazil, were used in the biosynthesis of silver nanoparticles. The synthesis of AgNPs were conducted utilizing the Penicillium spp. fermentation broth with 1 mL de AgNO3 (1mM). To conduct the characterization of AgNPs the 1,5 mL of reactional solution was submmited to the UV-Vis (200 and 800 nm) in 72, 144 and 216 h incubation, and fermentation broth without AgNO3 as control. Subsequently, the nanoparticles were submitted to evaluative assays to test their antimicrobial, effects. The nanoparticles presented inhibition of Escherichia coli and Staphylococcus aureus, with better results for the Gram negative bacterium. The results show that the green synthesis is a good method to produce metallic nanocomposites utilizing endophytic Penicillium spp.