ANOTAÇÃO DE SEQUÊNCIAS GÊNICAS DE ARROZ (Oryza sativa L.) IDENTIFICADOS NO GENOMA DA ESPÉCIE PELO BANCO DE DADOS GENÔMICOS Phytozome
ANOTAÇÃO DE SEQUÊNCIAS GÊNICAS DE ARROZ (Oryza sativa L.) IDENTIFICADOS NO GENOMA DA ESPÉCIE PELO BANCO DE DADOS GENÔMICOS Phytozome
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DOI: 10.22533/at.ed.1152310105
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Palavras-chave: genômica, banco genômico; marcadores moleculares
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Keywords: genomics, genomic bank; molecular markers
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Abstract: Rice (Oryza sativa L.) is the second most cultivated cereal in the world, being the food base of approximately 3 billion people, however, in the coming years, the reduction of the arable area and of biotic and abiotic factors affected the production of the crop. One of the alternatives to maintain the productive stability of the crop is the identification of genes related to traits of interest for rice improvement. This identification is carried out using biotechnological tools, such as molecular markers SNPs (Single Nucleotides Polymorphisms), which are defined as DNA markers that enable the identification of genes of interest to agriculture. Therefore, the objective of this work was to identify genes of interest in eight SNPs markers identified as polymorphic for the rice crop through in silico analysis, using the Phytozome genomic database. These genes were sensed based on their function, in addition to identifying the genomic sequence, CDS sequence (Coding DNA Sequence), peptide sequence and transcript sequence. As a result, it was possible to identify six SNPs within genes, located on six different chromosomes. Several functions were identified in the genes, such as hypothetical proteins, calmodulin related proteins, G proteins, ARK binding domain and protein expression. In rice, one of the most important functions is related to the expression of protein kinases located in the gene (LOC_Os04g45920, SNP S04_27195948) which, in turn, recognizes water stress in rice, representing sources of potency to be incorporated into new cultivars, through identification of these genes in materials such as traditional varieties. Therefore, it may be of interest to improve rice or other crops.
- LEONARDO FRANÇA DA SILVA