AMYLASES IN PROTEIN SECRETOME PROFILE FROM Aspergillus sp WITH POTENTIAL TO DECONSTRUCT INTEGRAL STARCH.

• DOI: 10.22533/at.ed.475180110

• Palavras-chave: Atena Editora

• Keywords: 15

• Abstract:

  Fungi have been strategically used as molecule producers of economic interest in the last decades, such as: enzymes, antibiotics, vitamins, amino acids and steroids; and the search for new enzymes or enzymatic systems that have the potential of deconstructing integral starch, has been one of the targets of our research group. In this aspect, the objective of this study was the investigation of the secretome of Aspergillus sp in the search of hydrolytic enzymes, using cassava starch (Manihot sculenta Crantz) as substrate. Twodimensional electrophoresis technique was applied to investigate the enzymatic profile and to verify its potential application as an enzymatic cocktail. The best enzyme production occurred in 96 hours of microorganism cultivation, also obtaining better amylolytic activity during this period, verifying the deconstruction of the starch and the generation of reducing sugars, mainly glucose in the reaction medium. In the overall profile of the enzymes performed by 2D electrophoresis, 29 protein spots were detected and will be identified after soon.

• Número de páginas: 15