A Diversidade da classificação de RNAs não-codificadores em bactérias

• DOI: 10.22533/at.ed.72719111117

• Palavras-chave: ncRNA, cis-encoded ncRNA, trans-encoded ncRNA, riboswitch.

• Keywords: ncRNA, cis-encodedncRNA, trans-encodedncRNA, riboswitch, CRISPR.

• Abstract:

  The genes that encode regulatory

  RNAs in prokaryotes - known as short RNAs

  (sRNAs) or non-coding sRNAs (ncRNAs).

  These molecules are transcribed in trans and

  in cis relative to the targeted RNA. They are

  located within the protein coding regions, in

  the intergenic regions of the genome and show

  signs of promoter and terminator sequences

  that are generally Rho-independent. The size

  of the ncRNA genes ranges from ~ 50 to ~

  500 nucleotides and several transcripts are

  processed by RNase with smaller end- products.

  These modulate the physiological responses

  through different mechanisms, either by RNARNA or RNA-protein interactions, and some of

  the interactions can be stabilized by the Hfq

  chaperone. The Riboswitches constitute another

  class of ncRNAs, that are located in the 5’UTR

  region of an mRNA and induce transcriptional

  regulation through their molecular interactions

  with linkers. CRISPR (Clustered Regularly

  Interspaced Short Palindromic Repeats) regions

  have been recently described in prokaryotes,

  which are based on repeated palindromic 

  sequences. Each replicate consists of small segments of “spacer DNA” taken from

  exposures prior to the isolation of a bacteriophage virus or exogenous plasmid. CRISPR

  can be defined as an immune system of resistance to exogenous molecules. 

• Número de páginas: 15