VIABILITY OF SPERMATOZOA REFRIGERATED AT 5 °C FOR 120 HOURS: EFFICIENCY OF DIFFERENT EXTENDERS IN THE MAINTENANCE OF BUFFER SEMEN FOR USE IN FTAI

• DOI: 10.22533/at.ed.9733102313077

• Palavras-chave: sperm longevity, computerized semen analysis, hyposmotic test.

• Keywords: sperm longevity, computerized semen analysis, hyposmotic test.

• Abstract:

  The objective of the present study was to verify the effectiveness of different extenders used for cooling buffalo semen in relation to maintenance of sperm longevity throughout processing. Seminal samples of 11 Murrah bulls aged 5 to 6 years were preserved at 5 °C for 120 hours in a passive refrigeration system (refrigerated counter) using TRIS extenders with 10% LDL (replacing egg yolk), Tolera D® (soy lecithin replacing egg yolk) and (Botu-Bov®). These samples were subjectively evaluated at T0 (post-collection), T1, T24, T48, T72, T96 and T120 hours post-dilution and refrigeration through computerized sperm movement analysis (CASA), plasmatic membrane integrity (Hyposmotic Test). Each male had his ejaculate analyzed for the physical and morphological characteristics of the semen, which were divided into three aliquots for each of the extenders. The values obtained were submitted to the Friedman Test (P<0.05). From the results achieved, it can be concluded that there was a better preservation of the samples kept under refrigeration with the Botu-Bov® extender at 5 °C/120 hours, providing greater protection against injuries and integrity of the spermatic membrane, when compared to the others. Thus, the Botu-Bov® extender can be an alternative for the use of refrigerated buffalo semen in AI and TAI for up to 48 hours.