β - OXIMA PROMOTES RELAXANT EFFECT IN RAT AORTA THROUGH KATP AND KIR POTASSIUM CHANNELS

• DOI: https://doi.org/10.22533/at.ed.1594992414117

• Palavras-chave: aorta, β-oxime, smooth muscle, nitric oxide.

• Keywords: aorta, β-oxime, smooth muscle, nitric oxide.

• Abstract:

  Oximes are called organic compounds whose general formula is RR'C=NOH, considering an organic substituent on R' and a hydrogen or any other organic group on R. They can be pharmacologically active when they release NO through oxidative cleavage of the C=NOH functional group and thus play an important role in the vascular system in relation to the vasorelaxation process. This study used male Rattus norvegicus which were sacrificed in accordance with the procedures approved by the UNIVASF Animal Experimentation Committee (Protocol No. 0003/111213). The organs were removed, dissected and sectioned into 3-5 mm rings, which were mounted in glass vats (10 ml) containing a medium conducive to keeping the organ alive. Contractions and relaxations were monitored by force transducers coupled to a digital acquisition system. The work presents pharmacological evidence to support the hypothesis that β-oxime has a relaxing effect reaching its maximum effect of 100% in a concentration-dependent and endothelium-independent manner in isolated rat aorta through the following mechanism of action, the relaxation of aortic smooth muscle promoted by β-oxime depends on potassium channels of the type KATP and KIR ; there is no dependence on the direct nitric oxide pathway, nor on the synthesis or participation ofs GC, with the relaxing effect promoted by this oxime. However, other mechanisms need to be investigated in order to better elucidate cell signaling.