DIRECT AND ELUATE COOMBS TEST FOR INVESTIGATION OF PERINATAL HEMOLYTIC DISEASE DUE TO ABO INCOMPATIBILITY

• DOI: 10.22533/at.ed.1592732222113

• Palavras-chave: direct antiglobulin test, eluate, perinatal hemolytic disease, maternal fetal ABO incompatibility, jaundice, phototherapy.

• Keywords: direct antiglobulin test, eluate, perinatal hemolytic disease, maternal fetal ABO incompatibility, jaundice, phototherapy.

• Abstract:

  Currently, ABO incompatibility has been a major cause of HPLD, where the mother's IgG antibodies, preferably those of blood group O, cross the placental barrier and attack fetal erythrocytes that contain A and/or B antigens. direct) is a test used to assist in this diagnosis, however, elution has been shown to be a test with greater sensitivity to assist in cases of DHPN. The aim of this study was to evaluate and compare the sensitivity and positive predictive value of TAD and elution in diagnosing DHPN. Materials and methods: TAD and freeze elution (LUI) were performed on samples of newborns from group A or B with mother from group O. Results: The results of this study identified a maternal fetal mother O incompatibility with fetus A of 80.3% and mother O with fetus B of 19.7%. The frequency of jaundice and need for phototherapy in the neonatal population was equivalent in the groups with LUI+/TAD- and LUI+/TAD+ and the negative predictive value of the eluate is 1. Conclusion: With this evaluation, it will be possible to early identify a possible ABO incompatibility as a cause hemolysis or jaundice, allowing better and faster management for patients. Thus, we conclude that, potentially, the eluate is an exam with better accuracy, especially in the sensitivity of the test and with a negative predictive value of 100%.