PRELIMINARY RESULTS IN THE EVALUATION OF THE CHEMOTHERAPY ACTIVITY OF BETA-SITOSTEROL IN A MODEL OF OSTEOSARCOMA IN RATS

• DOI: 10.22533/at.ed.813362304074

• Palavras-chave: Osteosarcoma, Benzopyrene, beta-sitosterol, chemotherapeutic, Murine model.

• Keywords: Osteosarcoma, Benzopyrene, beta-sitosterol, chemotherapeutic, Murine model.

• Abstract:

  Osteosarcoma (OS) is a malignant bone tumor, constitutes 75% in young patients, mainly affects bones of the lower extremities. There is no effective method to prevent this type of cancer and it can induce amputation. Beta-sitosterol (BS) is a plant compound. Studies have shown immunomodulatory, antimutagenic, anti-inflammatory, and antioxidant biomedical properties. Benzopyrene (BZP) is a hydrocarbon, capable of inducing OS. Considering this background, this research project was raised. Therefore, the objective was to determine, in a model of bone carcinogenesis in rats, the chemotherapeutic activity of beta-sitosterol in the development of osteosarcoma. Neoplastic lesions were induced by perifemoral administration of BZP. From the 7th to the 9th week BS was administered orally. The weight of the rats was recorded, blood samples were taken for genotoxicity studies; biochemical tests were performed to determine sedimentation rate (ESR), alkaline phosphatase (FA) and lactic dehydrogenase (DHL). At the ninth week, euthanasia was performed, femurs, tibiae, liver and lungs were removed for histopathological study.