REDUCTION OF BACULOVIRUS REPLICATION IN INSECT CELLS BY INFECTION AND/OR COINFECTION WITH A RECOMBINANT BACULOVIRUS CONTAINING THE NS1 PROTEIN OF INFLUENZA A VIRUS

• DOI: 10.22533/at.ed.813222216036

• Palavras-chave: NS1 protein, RNAi, insect cells, coinfection.

• Keywords: NS1 protein, RNAi, insect cells, coinfection.

• Abstract:

  This study aimed to evaluate the effect of the NS1 protein from Influenza A virus in stablished insect cells lines from different hosts (BM5 cells from Bombyx mori, UFL-AG-286 cells from Anticarsia gemmatalis, IPLB-SF21cells from Spodoptera frugiperda and BTI-Tn-5B1-4 from Trichoplusia ni) and with different virus susceptibility by coinfection of these cells with a recombinant baculovirus (vAcNS1) derived from the Autographa californica multiple nucleopolyhedrovirus isolate L-1 (AcMNPV L-1) and wild-type baculoviruses (AcMNPV L-1, Anticarsia gemmatalis multiple nucleopolyhedrovirus, isolate AgMNPV-2D, and Bombyx mori nucleopolyhedrovirus isolate, BmNPV-I-01). Polyhedra inclusion bodies (PIB’s) produced by wild type baculoviruses in coinfections with vAcNS1 was significantly reduced in all hosts insect cell lines tested. The production of budded virus was also decreased when the recombinant vAcNS1 was coinfected with all wild type baculoviruses. These results suggest that the NS1 protein expressed by vAcNS1 caused mostly adverse effects on the replication of baculoviruses in different cell lines.

• Número de páginas: 12