PRE-TREATMENT WITH PERACETIC ACID AND ENZYMATIC HYDROLYSIS OF SUGAR CANE BAGASS TO OBTAIN GLUCOSE

• DOI: 10.22533/at.ed.3173332325098

• Palavras-chave: Glucose; Cellulases; Lignocellulosic biomass.

• Keywords: Glucose; Cellulases; Lignocellulosic biomass.

• Abstract:

  Pretreatment and enzymatic hydrolysis are two important steps within the process of converting lignocellulosic biomass into glucose. Sugarcane bagasse (BCA) ground into particles smaller than 40 mesh was pre-treated with peracetic acid (APA) at concentrations of 5, 10 and 15% (v/v), in a time of 6 to 24h and temperature of 50 and 90 ºC in a solid:liquid ratio of 1:10. The pretreated BCA was subjected to hydrolysis with commercial enzymes in a solid:liquid ratio of 1:30, with 100 µL of cellulase mixtures and 100µL of Aspergilus niger laccase (807 U/g), in citrate-phosphate buffer (50 mM; pH 4.8) for 72h at 50 ºC under agitation at 150 rpm. The results of pre-treatment with APA showed an efficiency in the delignification of BCA of 71.1% and the glycan fraction increased from 44.78% to 53.06% in the solid residue of BCA recovered after pre-treatment carried out with APA 15 %, time of 24 hours at 50 ºC. Within a mass balance analysis, a solids recovery of 66.9% was observed for the pre-treatment stage with APA. Therefore, for every 100 g of pre-treated BCA, within the process, residue with 53.06g of glycan and 6.5g of lignin was recovered and for every 100g of BCA recovered after pre-treatment and enzymatically treated, it was possible obtain 56.88g of glycan hydrolyzed into glucose. Within these results, it is possible to conclude that acid pretreatment with APA and hydrolysis with commercial enzymes proved to be very efficient in converting glycan into glucose.