Can cellular cementum in bovine teeth alter the results of microleakage tests?

• DOI: https://doi.org/10.22533/at.ed.1594392418049

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• Abstract:

  Several studies use bovine teeth (DB) replacing human teeth (DH). We know that BD presents cellular cementum in the cervical region with a large concentration of cementocyte lacunae. This study evaluated whether this morphological difference has implications for microleakage results in cervical restorations. Cavity preparations (3mmx3mmx 2mm) were carried out on the buccal surface of 10 DH (3rd molar) and 10 DB (central incisor), with a cervical margin 1mm above the cementoenamel limit. Adhesive procedures (Scotch bond Multi-Purpose) were followed by the restoration with Filtek Z350XT. Polishing was carried out with aluminum oxide discs at low speed. Teeth were stored in distilled water at 37ºC/24h. The root apices were sealed and the teeth were waterproofed with cosmetic enamel, except over the restorations and 2mm below them. After drying, the teeth were immersed in 2% methylene blue at 37oC/24h. At the end, the teeth were washed/1min, and sectioned in the buccolingual direction. Microleakage was assessed with scores of 0-4 under a stereomicroscope (40X). The statistical analysis used was Mann-Whitney (=0.05). Kappa=1 confirmed agreement between examiners. The results showed that there was no significant difference between the groups (p=0.5205). However, it was observed that there was a difference in microleakage in the samples between the groups. In DH, microleakage always started from the tooth-restoration interface. However, in several samples in DB, microleakage began from the cellular cementum, below the cavity preparation, and continued to the pulp.