In vitro antimicrobial and cytotoxic effects of Talaromyces islandicus T101 from a polluted environment

• DOI: 10.22533/at.ed.813272220108

• Palavras-chave: Talaromyces islandicus; antibacteriano, citotoxicidade, produto natural.

• Keywords: Talaromyces islandicus; antibacterial; cytotoxicity; natural product.

• Abstract:

  Talaromyces islandicus TI01 was isolated from a marine-influenced environment that has been suffering for decades from anthropogenic actions in its body of water. Broth microdilution technique was performed to analyze the antimicrobial activity. For analysis of the cytotoxic activity, the MTT [3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide] assay was conducted. The chemical analysis of the extract was performed by LC/MS (liquid chromatography coupled to mass spectrometry). The minimum bactericidal concentration (MBC) of T. islandicus for E. coli ATCC 25922 and S. aureus ATCC 25923 was 1000 μg/ml. The minimum inhibitory concentration (MIC) for E. coli was 250 μg/mL and for S. aureus 500 μg/mL, respectively, whereas for C. tropicalis ATCC 1369 was 62.5 μg/mL. IC50 for breast cancer cell line (MCF-7) was 45.43 ± 1.657 μg / mL. The major compounds present in the extract were: Luteoskyrin (1) and N-GABA-PP-V (6-[(Z)-2-Carboxyvinyl]-N-GABA-PP-V) (2). The results show that T. islandicus TI01 has a prominent antibacterial activity against E. coli and S. aureus, making this fungi for the development of new food preservatives.