Green synthesis of magnetic nanoparticles of Cobalt Ferrite with Kiwi (Actinidia delicious) and its activity against the proliferation of Trypanosoma cruzi

• DOI: 10.22533/at.ed.317372315032

• Palavras-chave: -

• Keywords: Ferrite, Trypanossoma Cruzi, Actinidia delicious, nanoparticles.

• Abstract:

  Protagonist of a wide range of technological applications, nanoparticles (NPs) for reaching dimensions between 1 and 100 nm, provide characteristics and properties that stand out mainly in the pharmacological and biomedical areas. Skills such as hyperthermia and medication delivery system are examples of versatility, which has been the subject of many studies. The present work aims at the synthesis of cobalt ferrite nanoparticles, seeking the principles of green chemistry; characterize the nanoparticles produced and observe their antiproliferative behavior and activity in the culture medium of the protozoan T. cruzi,. To synthesize the nanoparticles respecting the principles of green chemistry, we use as raw material a fruit found in the Brazilian biodiversity: kiwi (Actinidia delicious), acquired in the local market. The components present in this fruit such as ascorbic acid, potassium, proteins, enzymes such as actinin, carbohydrates, among others, function as important structures for the interaction between elements with different charges, enabling the electrostatic effect through Coulomb's Law, as well as for the stabilization of the produced NPs. The cobalt ferrite NPs were synthesized by the sol-gel method, using MiliQ water as the solvent, where iron nitrate and cobalt were dissolved, which were subjected to heating to form cobalt ferrite (COFe₂O₄). The samples were centrifuged, dried in an oven (100ºC) and calcified in a muffle furnace (600ºC) for one hour. The characteristics related to the surfaces of the nanoparticles were analyzed using Scanning Electron Microscopy (SEM), which showed heterogeneity of particle sizes (5μm at 200 nm) as well as their aggregation. The crystallography analysis of X-Ray Diffraction, demonstrated the success in the formation of cobalt ferrite through the characteristic peaks present in the spectrum, and good crystallinity of the NPs, that is, a relatively organized internal structure. Subsequently, the sample was submitted to a biological assay study, in which the NPs were distributed in four different aliquots, which comprised four different concentrations (10, 20 and 50μg / mL), in which the 1mL of culture with T cruzi was added. This biological test was followed up for eight consecutive days, providing us with enough data to analyze the behavior of the protozoan in the presence of NPs. The biological assay showed significant antiproliferative activity for the 50μg / ml concentration. Which allows us to conclude that the aggregation confirmed by SEM, and the size of the NPs estimated by the DRX, did not prevent the antiproliferative activity of T. cruzi.